One possible explanation is that WJ68 possesses two copies of the ars1 operon and T. arsenivorans has two copies of the Acadesine cell line ars2 operon. Alternatively, the higher resistance capacities of T. arsenivorans, Thiomonas sp. 3As, and WJ68, as compared to Ynys1 and T. perometabolis may be due to greater As(III) oxidation capaCity of these strains. The arsenic response observed in T. arsenivorans
and 3As revealed that the proteins involved in arsenic resistance (ars genes) were more highly expressed in the presence of arsenic, as shown previously for H. arsenicoxydans [25, 28], Pseudomonas aeruginosa [29] and Comamonas sp. [30]. Therefore, such a feature seems to be a common arsenic response. In H. arsenicoxydans, other proteins that were shown to be more abundant in the presence of arsenic were involved in oxidative stress, DNA repair and motility. In this study, such proteins (hydroperoxide reductase, methyl-accepting chemotaxis protein, PilM) were induced in Thiomonas sp. 3As whereas in T. arsenivorans, only general stress proteins were induced. These observations suggest that the response to the stress induced learn more by arsenic involves different regulatory mechanisms in 3As and T. arsenivorans. Contrary to this arsenic-specific response, the other arsenic-regulated proteins identified in the Thiomonas strains did not share a similar expression pattern with other arsenic-resistant
bacteria. Thus it appears that while there may be a common arsenic response between all the bacteria, the general metabolism may be differentially adapted to each environment from which these strains originated. In particular, T. arsenivorans Roflumilast has unique traits in terms of arsenic, carbon and energy metabolism that distinguish it from the other strains examined. Thiomonas arsenivorans can grow autotrophically using either As(III) or thiosulfate as the sole energy source. Surprisingly, the differential
protein expression analysis revealed that even in the presence of yeast extract, proteins involved in CO2 fixation through the Calvin-Benson-Bassham cycle and enzymes involved in the glycolysis/neoglucogenesis were expressed. In addition, it was shown in the present study that T. arsenivorans induces expression of carbon fixation-specific enzymes in the presence of arsenic. This Talazoparib solubility dmso observation was correlated with an increased CO2 fixation efficiency when arsenic concentration increased. This suggests that an increase in cbb genes expression in the presence of arsenic improves its capaCity to fix CO2. On the other hand, the opposite observation was seen with Thiomonas sp. 3As. Therefore, the proteomic results obtained from the present study suggest that these two Thiomonas strains react differently to their arsenic-contaminated environments. The other differences observed concern DNA metabolism, transcription and protein synthesis. It appears that, in the presence of arsenic, T.