32-34 Indeed, HSCs secrete Ang1 to promote formation of junctional complexes between LECs, a key step for angiogenesis and vascular restructuring within a mechanically stressed fibrotic microenvironment.1, 18, 35 Importantly, the capillary response regulated by Ang1 in diseased liver in vivo appears to be congruent with molecular mechanisms described here. For example, although normal liver sinusoids are characterized by a discontinuous phenotype, in cirrhosis these delicate vascular structures undergo what is commonly referred to as “capillarization,” with more durable stellate

cell coverage of more closely interconnected http://www.selleckchem.com/products/ly2157299.html endothelial cells. These phenotypic changes coincide with known functions of Ang1 as a stabilizer Alvelestat mouse of vessels.36 Therefore, our results may also help to explain how sinusoidal vasculature adopts distinct phenotypic changes in cirrhosis and use this knowledge for designing future therapeutic interventions targeting this pathway. In total, this study underscores the importance of considering both vasculature and matrix as combined therapeutic targets of therapies aimed to ameliorate cirrhosis

and its complications. We thank Helen Hendrickson for managerial support in the laboratory and Terri Johnson for secretarial assistance. Additional Supporting Information may be found in the online version of this article. “
“Aim:  The onset of depression symptoms during pegylated interferon α plus ribavirin (PEG-IFN/RBV) 上海皓元 combination therapy has led to treatment discontinuation in some cases. In the present study, we conducted a questionnaire survey during treatment to determine whether natural human interferon β plus ribavirin (IFNβ/RBV) therapy is associated with a lower incidence of depression symptom onset compared with PEG-IFN/RBV therapy. Methods:  Seventy-seven patients with chronic hepatitis C received PEG-IFN/RBV (PR) or IFNβ/RBV (FR) therapy. A questionnaire survey was administered at the start of treatment, and at 4 and 12 weeks, using the Beck Depression Inventory

II (BDI-II) and the Pittsburgh Sleep Quality Index (PSQI). Results:  BDI-II scores in the PR group increased at 4 and 12 weeks, but remained unchanged in the FR group. At 12 weeks, the mean BDI-II score and incidence of abnormalities with a BDI-II score of ≥14 were significantly lower in the FR group than in the PR group. BDI-II scores during IFNβ/RBV therapy in 11 patients currently using antidepressants remained unchanged up to 12 weeks. None of these 11 patients required addition or dose increases of antidepressants, and there was no evidence of worsened depression symptoms. Nine PR patients had BDI-II scores of ≥14 and PSQI scores of ≥11 at 12 weeks. Conclusions:  IFNβ/RBV therapy was associated with a lower incidence of depression symptom onset during treatment.

Surgical treatment of this complication is accompanied by a high mortality rate. Endoscopic or radiographic

interventions are preferable. Methods: We described a case in which a fistula on descending colon, due to necrotizing pancreatitis, was effectively treated using the over-the-scope Doxorubicin price clip system (OTSC – system; Ovesco Endoscopy AG, Tubingen – Germany). Results: A 76-years old woman patient, with a known history of gallstones, hospitalized for acute abdominal pains, vomiting, fever and jaundice. Laboratory tests showed severe anaemia, leukocytosis and elevated levels of liver and pancreatic enzymes, VES and

CRP. The patient was subjected an emergency laparotomy. Surgical exploration revealed a pattern of exudative-necrotizing pancreatitis associated with diffuse peritonitis and an abundant abdominal and pleural effusion. The hydropic gallbladder contained multiple stones and the intraoperative cholangiography exluded the presence of stones or abnormality in the bile ducts. At first, a cholecystectomy was performed and trans-cystic drainage was inserted and then, an accurate toilette of peritoneal cavity was maked. Multiple drainages were placed in the pancreatic area with necrotic/purulent and blood materials to leak out, for some days. Even 上海皓元 so, on the EPZ015666 15th post-operative day, a great peri-pancreatic infected collection, extended to spleen lodge, developed. Furthermore, during second look, further drainages were placed and many daily washes were performed. Subsequently,

a next radiologic examination discovered a fistula involving the peri-pancreatic abscess and descending colon. Therefore, the patient was transferred to our Unit of Gastroenterology and Digestive Endoscopy, in steady-state conditions and contrast-enhanced TC was performed. The drained fluid through the percutaneous drainage showed the communication with descending colon. A lower gastrointestinal endoscopy confirmed the presence of the retroperitoneocolonic fistula. After administration of methylene blue, the drained fluid showed blue staining in the anterior abdominal wall and the communication with colon. An attempt to seal the perforation endoscopically was performed using the OTSC system and the lesion was closed with one clip. The fistula showed a good healing such as reported by subsequent radiologic examinations. The drainages were removed gradually and the patient was discharged in some weeks later.

All training sessions were carried out under the supervision of exercise specialists. Participants exercised for 60 minutes per session at Decitabine manufacturer 60%-65% of heart rate reserve, as estimated by the Karvonen formula.[11] Aerobic activities were performed on treadmill, cycle, or elliptical machines and participants were free to change the cardiovascular equipment used from one session to the next. Heart rate monitors were used to standardize exercise intensity. Participants performed nine different exercises involving the major muscle groups on weight machines (chest press, shoulder press, vertical traction, leg press, leg extension, leg curl, abdominal crunch) and free weight

(biceps, abdominal). After a learning phase, participants performed 3 series of 10 repetitions at 70%-80% 1-RM, with 1 minute of recovery between series. All participants met a single nutritionist for nutritional counseling at least 2 months before the study. Participants were encouraged to follow a healthy diet, according to standard recommendations for people with type 2 diabetes. Thereafter, participants were instructed to maintain

their baseline calorie intake by consuming self-selected Selleck Roxadustat foods. The same nutritionist met the participants of both groups on two occasions, just before and at the end of the intervention, to record and analyze their 3-day food recalls. Weight was recorded on an electronic scale (Tanita BWB-800, MA, USA), height was measured with a Harpenden stadiometer, and BMI was calculated as weight (kg)/height[2] (m). Total body fat mass was evaluated by dual-energy x-ray absorptiometry (DXA) using a total body scanner (QDR Explorer W, Hologic, Waltham, MA). HbA1c was measured

上海皓元医药股份有限公司 by a Diabetes Control Complications Trial (DCCT)-aligned method, with an automated high-performance liquid chromatography analyzer (Bio-Rad Diamat, Milan, Italy). Serum lipids and transaminase levels were determined by standard laboratory procedures (DAX 96; Bayer Diagnostics, Milan, Italy). Low-density lipoprotein (LDL)-cholesterol was calculated using the Friedewald equation.[12] MRI was used to measure the amount of fat at the level of the liver and abdomen. A single radiologist, who was blinded to participants’ clinical details, performed all MRI examinations by using a 1.5-T magnet (Magnetom Vision; Siemens Medical, Erlangen, Germany). Liver fat accumulation was measured by comparing the in- and out-of-phase images of tissues according to Dixon’s two-point method.[13-15] To obtain these data, patients were positioned supine using a phased array coil. Axial T1-weighted gradient echo images and in-phase and out-of-phase images were obtained from the upper abdomen and the thighs. Scan parameters were the following: TR/TE 160/2.l msec (out-of-phase) and 4.2 msec (in-phase), flip angle 80°, slice thickness 8 mm with 1 mm interslice gap. Image postprocessing was performed using a workstation (MV-1000; Siemens Medical).

9 showed a positive correlation between the 25-hydroxyvitamin D concentration and insulin sensitivity, and subjects with hypovitaminosis D were found to be at higher risk for insulin resistance and metabolic syndrome. These findings make it rational for us to infer that the prevalence of vitamin D insufficiency and hypovitaminosis D among African Americans

can increase their risk of insulin resistance. This may account in part for the fact that despite the lower levels of intraperitoneal and liver fat in African Americans, their prevalence of insulin resistance is similar to that of Hispanics, who possess the highest intraperitoneal and liver fat levels.1 Therefore, vitamin D insufficiency www.selleckchem.com/products/Everolimus(RAD001).html and hypovitaminosis D in African Americans may provide useful clues for understanding their high prevalence of insulin resistance. Hong-Fang Ji Ph.D*, * Shandong Provincial

Research Center for Bioinformatic Engineering and Technique, Shandong University of Technology, Zibo, People’s Republic of China. “
“Primary hepatic lymphoma is a rare lymphoma that is confined to the liver with no evidence of involvement of the spleen, lymph nodes, bone marrow or other lymphoid structures. The most common presenting symptom is discomfort or pain in the right upper quadrant of the abdomen. This is sometimes accompanied by malaise, anorexia, weight loss, fever, nausea and vomiting. There are also case reports of fulminant hepatic 上海皓元 failure with hepatic encephalopathy. The

typical radiological appearance is that of BMN 673 nmr a solitary lesion although multiple lesions or diffuse liver infiltration have also been described. In most patients, the differential diagnosis will include primary and secondary hepatic tumors as well as systemic lymphoma with secondary hepatic involvement. Case reports have raised the possibility of an association between primary hepatic lymphoma and hepatitis C but, for most patients, the pathogenesis remains unclear. We describe two patients with primary hepatic, B-cell lymphomas who had negative serological tests for both hepatitis B and C. The first patient was a 92-year-old woman who was admitted to hospital because of epigastric pain, nausea, vomiting, weight loss and fever over the preceding 6 months. Physical examination revealed enlargement of the left lobe of the liver and mild ascites. A contrast-enhanced computed tomography (CT) scan of the abdomen showed multiple hypodense lesions in the left and right lobes of the liver (Figure 1). She also had thrombosis of the inferior vena cava. A liver biopsy under ultrasound control revealed a neoplastic population of intermediate and large lymphoid cells with immunochemical stains that were positive for CD20 and bcl-6. Other investigations including a bone marrow biopsy were normal. She declined therapy and died after 3 months.

“
“Severe recent declines of amphibians around the world

have highlighted the need to identify factors that affect their population dynamics and viability. This study used a long-term (>30 years) dataset collected for a British population of natterjack toads Bufo calamita, a rare and endangered species in much of northern Europe. Modelling was employed to test a series of hypotheses concerning the effects of anthropogenic (conservation management) and climatic factors on toad demographics. The best models accounted for >72% of the variance in population size, as judged by spawn string counts, between 1975 and 2007. Conservation management (pond creation) was important, as were spring and summer climate variables relating to larval survival, and winter conditions associated with hibernation buy ITF2357 mortality. The implications

of trends associated with future climate change are also considered. “
“Predator selectivity for age and sex classes has large implications for their impact on prey populations. We FK506 nmr examined whether the Eurasian lynx Lynx lynx selects specific sex and age categories of roe deer Capreolus capreolus, and if this selection pattern differs between summer and winter. Data on sex and age of 194 roe deer killed by 44 VHF- and GPS-marked lynx were collected in southern Norway from 1995 to 2010. The sex and age distribution of the roe deer population was estimated using demographic parameters

estimated from radio-collared roe MCE公司 deer in the study area. We found that lynx selection differed between summer and winter. In both seasons, lynx selected adult roe deer of both sexes. In summer, there was a clear selection against yearlings, but in winter, lynx selected male yearlings. Compared with the availability, fawns of both sexes were under-represented during summer. Male and female lynx did not differ in their prey selection, but yearling lynx tended to kill a larger proportion of roe deer fawns than older lynx. We argue that seasonal differences in behaviour, activity and habitat use by roe deer may explain this variation in lynx selection patterns, supporting the view that prey selection is affected by the life cycle stage of both the predators and the prey. “
“Population-level distribution strategies, such as migration, nomadism or residency, form often as a result of spatio-temporal resource dynamics. While commonly a species will adopt a single strategy across its range, occasionally multiple strategies can be observed. In Australia, the eastern grass owl Tyto longimembris is considered nomadic over most of its range. However, resident populations have been reported along the eastern coastal zone. We collected and analysed regurgitated pellets of a coastal resident population across three seasons in a single year.

Biomechanical function of the knee and ankle during level walking, lateral gastrocnemius anatomical selleck chemical cross-sectional area, thickness, width, fascicle length and pennation angle and ankle plantar flexor muscle

strength were recorded in 19 typically developing boys aged 7–12 years and 19 age-matched haemophilic boys with a history of ankle joint bleeding. Associations between gait, strength and architecture were compared using correlations of peak gait values. Haemophilic boys walked with significantly larger (P < 0.05) ankle dorsi flexion angles and knee flexion moments. The ankle plantar flexor muscles of haemophilic boys were significantly weaker and smaller when compared to typically developing peers. In the typically developing boys there was no apparent association between muscle architecture, strength and walking patterns. In haemophilic boys maximum muscle strength and ACSA normalized torque of the ankle GSK3235025 manufacturer plantar flexors together with the muscle width, thickness, fascicle length and angulation (P < 0.05) were associated with motion at the ankle and peak moments at the knee joint. Muscle strength deficits of the ankle plantar flexors and changes in muscle size and architecture

may underpin the key biomechanical alterations in walking patterns of haemophilic boys with a history of ankle joint bleeding. “
“Erik Adolf von Willebrand (VW) was born in 1870 in the city of Vaasa in Finland (Figs. 1 and 2). Although the family was socially active and class conscious, VW’s upbringing was austere by modern standards. He attended Vaasa Lyceum especially excelling in chemistry, botany and zoology. In the summers, he trekked widely collecting botanical, lepidopterological and ornithological specimen and in the winters he toured the frozen 上海皓元 Gulf

of Bothnia. After gaining his baccalaureate in 1890, VW enrolled at the University of Helsinki and before gaining his license in 1896, he spent the summers of 1894 and 1895 on the Åland islands (Fig. 2) as a junior spa physician. However, at this time, there is no evidence that he would have encountered the disease he later was to describe. After graduation, in 1897, VW was attached as assistant physician to the Department of Medicine at the Deaconess Hospital in Helsinki where Professor Ossian Schauman, an eminent haematologist, supervised VW’s dissertational work on changes in blood cell count following venesection. Other early haematological studies of VW included regeneration of blood in anaemia and a novel method for staining of blood smears using eosin and methylene blue. After completing his dissertation in 1899, VW took up the position as chief physician at the Heinola Spa, and the focus of his work changed to applied physiology. Between 1900 and 1906 VW also held positions at the Departments of Anatomy and Physiology at the University of Helsinki.

Methods: ESTD was attempted in six consecutive patients with gastric neoplasia with clinical indications for endoscopic resection from August to November 2012 in our institute. For

the gastric superficial neoplasia, the large ones were defined as lesions with length ≥25 mm and width ≥30 mm. Routine ESD equipment and accessories were used in the operation. After the margin of the lesion was marked, one submucosal tunnel created by submucosal dissection from oral incision to anal incision. Finally, bilateral dissection was performed to remove the lesion completely. After completion of ESTD, preventive coagulation was routinely performed R428 ic50 for all visible vessels on the artificial ulcer using hemostatic forceps or argon plasma coagulation. Results: For the six lesions, the maximum diameter was from 40 mm to 50 mm (mean 44 mm). The operation time ranged from 35 minutes to 98 minutes (mean 73 minutes). En bloc resection was achieved without complications in all the lesions. After ESTD, the pathology demonstrated that two lesions were high-grade DAPT concentration intraepithelial neoplasia and four lesions were gastric cancer, which were resected

completely but one with positive basal margins. The patient with residual lesion was performed surgery 12 days later, and then the pathology demonstrated no residual cancer. Conclusion: ESTD technique is feasible and appears to be efective and safe for removal large gastric superficial neoplasia. Its efficacy and safety still need to be further confirmed by larger, comparative studies. Key Word(s): 1. ESTD; 2. gastric neoplasia; Presenting Author: YING KIT LEUNG Corresponding Author: YING KIT LEUNG Affiliations: Precious MCE Blood Hospital Objective: performance of SBE uses the hooking maneuver to anchor the tip of scope to the mucosa and is often unsuccessful because the mucosa is slippery resulting in sliding back of the scope during advancement of the overtube. Double balloon enteroscopy uses an additional balloon at the tip of the scope to achieve anchorage but is also often not very secure. The aim of this

study is to use a novel method to prevent the scope from slipping back during insertion of the overtube when the overtube balloon is deflated. Methods: We use a cap that can be fitted snugly to the tip of the scope. The cap obscures about 5% of the visual field during the procedure. When the overtube balloon is to be deflated prior to overtube advancement over the shaft of the scope, the tip of the scope is impacted against the small bowel mucosal and maximal suction applied. The tip of the scope is firmly adhered to the mucosa as negative pressure is applied, preventing the scope from slipping. The suction is released when the balloon is reinflated. The cycle is repeated during advancement of the scope. Results: A total of thirteen patients underwent SBE in this manner, twelve retrograde and one antegrade. Indications of the procedure: abdominal pain, bleeding, ulceration and Crohn’s disease.

Methods: A total

of 159 consecutive patients with chronic liver failure were included in the study and divided into two groups (death group and survival group) according to the prognosis. The levels of total bilirubin (TBIL), serum creatinine (Cr), prothrombin time (PT), PT international normalized ratio (INR), Serum sodium(Na), age, MELD, MELD- Na and iMELD were calculated respectively and the comparative analysis was performed. Areas under the receiver operating characteristic curve (AUC-ROC) of MELD, MELD-Na and iMELD were used to assess the prognosis in patients with chronic liver failure. Results: The values of age, TBIL,, INR, MELD, MELD-Na and iMELD were significantly higher in death group than those in survival group (P < 0.01). The serum level of Na+ was significantly lower in death group than MLN0128 datasheet that of survival group (P < 0.01). The mortality of liver failure was higher in patients with the increased scores of MELD, MELD-Na

and iMELD. The area under curve (AUC) values generated by the ROC curves was no difference respectively(P > 0.05) for MELD score (AUC = 0.691),MELD-Na score (AUC = 0.690) and iMELD score (AUC = 0.674). Conclusion: The cut-off scores of three systems were 25.8 (MELD), 31.0(MELD-Na) and 53.5(iMELD) respectively, which could discriminate higher and lower mortality accurately. Key Word(s): 1. Liver Disease; 2. Liver Failure; 3. Treatment; 4. End-stage; Presenting Author: JAE HYUN KIM Additional Authors: WON selleck chemicals MOON, SEUN JA PARK, MOO IN PARK, SUNG EUN KIM Corresponding Author: WON MOON Affiliations: Department of

Gastroenterology Objective: Endoscopic ultrasonography (EUS) is helpful to evaluate the depth of tumor invasion and determine the treatment strategies of rectal neuroendocrine tumors (NETs). The aim of this study was to clarify the clinical impact of EUS for 10 mm or less in diameter of rectal NETs. Methods: Between June 2006 and March 2013, a total of 76 rectal NETs treated at our hospital were reviewed, retrospectively. Total 81 patients of rectal NETs were included and 6 patients were excluded for their tumor size (>10 mm) on histologic evaluations. 1 patient had two synchronous rectal NETs. The depth of tumor invasion was evaluated by EUS. All MCE of the lesions were resected by endoscopic submucosal resection with band-ligation (ESMR-L) and were analyzed histologically. Lymph node metastasis and distant metastasis of the tumors were evaluated by abdominal CT. Results: The mean size of the resected tumors were 4.7 mm (range 1.0–10 mm) on histologic evaluations and were 6.6 mm (range 3.0–15 mm) on colonoscopic findings. On EUS findings, all of the 76 lesions confined to the submucosa and invasion of the tumors were within the upper two-thirds of the submucosa.

18 Nonbound HCVcc were removed by buy GW-572016 washing of cells with phosphate-buffered saline, and cell bound HCV RNA was then quantified by reverse-transcription polymerase chain reaction.18 HCV cell-to-cell transmission was assessed as described.2, 24 Producer Huh7.5.1 cells were electroporated with Jc1 RNA33 and cultured with naïve target Huh7.5-GFP cells in the presence or absence of anti–SR-BI or control mAbs. An HCV E2-neutralizing antibody (AP33, 25 μg/mL) was added to block cell-free transmission.24 After 24 hours of coculture, cells were fixed with paraformaldehyde, stained with an NS5A-specific antibody (Virostat),

and analyzed via flow cytometry.2, 24 Cell spread was assessed by visualizing Jc1-infected Huh7.5.1 cells by immunoflorescence using anti-NS5A (Virostat) and anti-E2 (CBH23) antibodies as described.2 HDL was labeled using Amersham Cy5 Mono-Reactive Dye Pack (GE Healthcare). Unbound Cy5 was removed by applying labeled HDL on illustra MicroSpin G-25 Columns (GE Healthcare). Blocking of Cy5-HDL binding with indicated reagents was performed for 1 hour at room temperature prior to Cy5-HDL binding for 1 hour at 4°C on 106 target cells. Selective HDL-CE uptake and lipid efflux assays were Erlotinib performed as described.23, 34 HDL-CE uptake was

assessed in the presence or absence of anti–SR-BI mAbs (20 μg/mL) and 3H-CE-labeled HDL (60 μg protein) for 5 hours at 37°C. Selective uptake was calculated from the known specific radioactivity of radiolabeled HDL-CE and is denoted in

μg HDL-CE/μg cell protein. For lipid efflux assay, Huh7 cells were labeled with 3H-cholesterol (1 μCi/mL) and incubated at 37°C for 48 hours as described.23, 35 Cells were incubated with anti–SR-BI mAbs (20 μg/mL) for 1 hours prior to incubation with unlabeled HDL for 4 hours. Fractional cholesterol efflux was calculated as the amount of label obtained in the medium divided by the total in each well (radioactivity in the medium + radioactivity in the cells) regained after lipid extraction from cells. Unless otherwise stated, MCE data are presented as the means ± SD of three independent experiments. Statistical analyses were performed using a Student t test and/or Mann-Whitney test; P < 0.01 was considered statistically significant. To further explore the role of HCV–SR-BI interaction during HCV infection, we generated five rat and three mouse monoclonal antibodies (mAbs) directed against the human SR-BI (hSR-BI) ectodomain (Table 1). These antibodies bound to endogenous SR-BI on human hepatoma Huh7.5.1 cells and primary human hepatocytes but did not bind to mouse SR-BI (mSR-BI) expressed on rat BRL cells (Fig. 1A,B and Supporting Fig. 1). Three rat mAbs (QQ-4A3-A1, QQ-2A10-A5, and QQ-4G9-A6) and one mouse mAb (NK-8H5-E3) significantly (P < 0.01) inhibited HCVcc infection in a dose-dependent manner with 50% inhibitory concentrations (IC50) between 0.

The diagnoses of chronic hepatitis B and liver failure were based on previously described criteria.18, 19 Informed consent was obtained before the study. Studies began after 1 week of the same medical treatments (i.e., reduced glutathione, glycyrrhizin, ademetionine, polyene phosphatidylcholine, Alectinib order alprostadil, and human serum albumin) were performed on all patients. This time point was used as a baseline. All patients were

informed about the process of autologous transplantation of MMSCs and volunteered to receive this treatment. A total of 53 patients (group A) accepted the protocol, and transplantations were performed within 3 days after 1 week of the medical treatments mentioned above. In addition, the medical treatments mentioned above were continued throughout the study for all patients. A total of 105 patients

(group B) matched for age, sex, and some biochemical indexes, including alanine aminotransferase (ALT), albumin (ALB), total bilirubin (TBIL), prothrombin time (PT), and Model for End-Stage Liver Disease (MELD), served as controls (Table 1). In addition, the patients in each group were divided into subgroups of patients with or without cirrhosis: 39 and 77 patients with cirrhosis in subgroups A1 and B1 and 14 and 28 without cirrhosis in subgroups A2 and B2 (no significantly differences were found). Cirrhosis was diagnosed by the evidence of a small, nodular liver, as shown by ultrasound, computerized tomography (CT), and http://www.selleckchem.com/products/mi-503.html magnetic resonance (MR), with the exclusion of primary biliary cirrhosis and cirrhosis caused by schistosome. Inclusion criteria consisted of 15-75 years of age, agreement to informed consent, and a diagnosis of hepatitis B–induced liver failure.18, 19 Exclusion criteria included pregnant

and lactating women, antiviral or immunomodulatory therapy within 6 months before surgery, presence of other factors causing active liver diseases (e.g., autoimmune 上海皓元医药股份有限公司 diseases, drug-induced liver disease, alcoholic liver disease, inherited metabolic liver diseases, etc.), concomitant human immunodeficiency virus (HIV) infection or congenital immune deficiency diseases, proven liver cancer or other malignancies, severe diabetes, autoimmune diseases, other important organ dysfunctions (e.g., kidney dysfunction), concomitant infection (e.g., fever, leukocytosis or neutrophilia, and manifestations of abdominal, biliary tract, or lung infection) or other serous complications (e.g., hepatic encephalopathy, gastrointestinal bleeding, etc.), intolerance to the medical treatments mentioned above, and patients having received, or who would receive, bioartificial liver support therapy or liver transplantation. Our study is registered at ClinicalTrials.gov (NCT00956891), with the registered name of “Long-Term Follow-up of Liver Failure Patients Who Received Autologous Mesenchymal Stem Cells (MSCs) Transplantation.