PXEis a rare genetic condition. It is associated with calcification of elastinfibers and is characterized by skin, eye, and cardiovascular complications. Our patient was previously treated for retinal and gastrointestinal hemorrhage and coronary artery disease, and is under surveillance for cerebral aneurysms. Five reports in the published literature have described aneurysms in patients with PXE, but, to our knowledge, this is the first report of a patient with PXE and renal artery aneurysm. The literature on PXE and aneurysms is reviewed. (J Vasc OICR-9429 supplier Surg 2013;57:842-4.)”
“Plant seed oil bodies, subcellular
lipoprotein inclusions providing storage reserves, are composed of a neutral lipid core surrounded by a phospholipid monolayer with several integrated proteins that play a significant role in stabilization of the particles and probably also in lipid mobilization. Oil bodies’ proteins are generally very hydrophobic, due to the long uncharged sequences anchoring them into the lipid core, which makes them BTSA1 ic50 extremely difficult to handle and to digest successfully. Although oil bodies have been intensively studied during last decades,
not all their proteins have been identified yet. To overcome the problems connected with their identification, a method based on SDS-PAGE, in-gel digestion and LC-MS/MS analysis was used. Digestion was carried out with trypsin and chymotrypsin, single or in combination, which increased significantly the number of identified peptides, namely the hydrophobic ones. Thanks to this methodology it
was possible to achieve an extensive coverage of proteins studied, to analyze their N-terminal modifications and moreover, to detect four new oil bodies’ protein isoforms, which demonstrates the complexity of oil bodies’ protein composition.”
“We report the case of a 26-year-old woman who presented with embolic stroke from left common carotid artery compression by a gigantic clavicular osteochondroma. To our knowledge, this is the only such case described in the literature. The other particularity of this case is the delayed appearance of this childhood tumor. Surgery was successful, with a satisfying outcome. Cytidine deaminase (J Vasc Surg 2013;57:845-7.)”
“Purpose: The Quantitative Assay Database (QuAD), http://proteome.moffitt.org/QUAD/, facilitates widespread implementation of quantitative mass spectrometry in cancer biology and clinical research through sharing of methods and reagents for monitoring protein expression and modification.
Experimental design: Liquid chromatography coupled to multiple reaction monitoring (LC-MRM) mass spectrometry assays are developed using SDS-PAGE fractionated lysates from cancer cell lines.