The relationship of H. pylori and GERD is controversial. This study was performed to understand the role of H. pylori virulent genes in GERD

in North India. Methods: This study was conducted among 405 patients who visited the OPD of Yashoda Super Specialty Hospital and agreed to participate in the study. The subjects included patients (n = 342) and controls (n = 63), (those who undergoing endoscopy for reasons other than GERD). The genotyping of the cagA and vacA gene was done by PCR in 63 patients (including GERD (n = 21), NERD (n = 27), antral gastritis (n = 4), Peptic Ulcer (n = 2), Fungal esophagitis (n = 1), Non-ulcer dyspepsia (n = 3) and control patients (n = 5). GERD was diagnosed by FSSG questionnaire Atezolizumab research buy (Score >7) and endoscopic evidence of esophagitis; NERD was diagnosed

if FSSG score >7 and normal endoscopy. Results: H. pylori was cultured from 63 (15.6%) patients. The MAPK Inhibitor Library cell line average age of 63 patients included in the study was 44.9 yr. + 16.6 yr and the M : F ratio was 1:0.615. Of the 63 H. pylori strains, 27 (42.9%) strains were cagA positive while 36 (57.1%) strains were cagA negative. Correlation of the disease status with the genotype. It was observed that cag A positive strains of H. pylori were less prevalent in Reflux esophagitis (8/21; 38.1 %) and Nonerosive reflux esophagitis groups (12/27; 44.4%) than cag negative in Reflux esophagitis (13/21; 61.90 %) strains(Table 1). However, these differences did not achieve significance value (p > 0.05). Further, it was found that the cag negative strains were significantly more (p < 0.05) associated with less severe Reflux esophagitis [(Grade A/B); 13/13 100%] while cag positive strains caused more severe esophagitis [(Grade C/D); 3/8 37.5] CHI SQAURE TEST VALUE = 5.69, p value = .017 (p < 0.05), significant. Conclusion: cag A positive strains of Helicobacter pylori may be causative for severe GERD. Key Word(s): 1. cagA; 2. Helicobacter pylori;

3. Reflux esophagitis; 4. India; Table 1: Relationship between Disease Status and Genotype DISEASE STATUS TOTAL NO. OF PATIENTS NO. OF PATIENTS cagA negative/cagA positive Reflux oesophagitis 21 13 (61.90%)/8 (38.09%) (LA grade A/B/C/D)     NERD 27 15 (55.55%)/12 (44.44%) NUD 3 1 (33.33%)/2 (66.66%) Peptic – DU/GU 2 0/2 (100%) Antral 上海皓元医药股份有限公司 gastritis 4 4 (100%)/0 Fungal Esophagitis 1 1 (100%)/0 Control 5 2 (40%)/3 (60%) Presenting Author: GAILING WU Additional Authors: YU LAN Corresponding Author: GAILING WU Affiliations: gastroenterology; gastroentrology Objective: It is difficult to select the therapy scheme for Helicobacter pylori (H. pylori) infected patients with penicillin allergy. Since the tetracycline is seldom to be obtained, the furazolidone’s adverse effect is high, the antibiotics that used for treating the H. pylori infected patients are clarithromycin, tinidazole, levofloxacin and so forth.