Staining Analysis Immunoreactivity for Cx26 was considered to be positive if distinct staining of the cytoplasm was observed in at least 10% of the tumor cells (Fig. 1) and P53 was considered to be positive if distinct staining of the nuclei was observed {Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|buy Anti-infection Compound Library|Anti-infection Compound Library ic50|Anti-infection Compound Library price|Anti-infection Compound Library cost|Anti-infection Compound Library solubility dmso|Anti-infection Compound Library purchase|Anti-infection Compound Library manufacturer|Anti-infection Compound Library research buy|Anti-infection Compound Library order|Anti-infection Compound Library mouse|Anti-infection Compound Library chemical structure|Anti-infection Compound Library mw|Anti-infection Compound Library molecular weight|Anti-infection Compound Library datasheet|Anti-infection Compound Library supplier|Anti-infection Compound Library in vitro|Anti-infection Compound Library cell line|Anti-infection Compound Library concentration|Anti-infection Compound Library nmr|Anti-infection Compound Library in vivo|Anti-infection Compound Library clinical trial|Anti-infection Compound Library cell assay|Anti-infection Compound Library screening|Anti-infection Compound Library high throughput|buy Antiinfection Compound Library|Antiinfection Compound Library ic50|Antiinfection Compound Library price|Antiinfection Compound Library cost|Antiinfection Compound Library solubility dmso|Antiinfection Compound Library purchase|Antiinfection Compound Library manufacturer|Antiinfection Compound Library research buy|Antiinfection Compound Library order|Antiinfection Compound Library chemical structure|Antiinfection Compound Library datasheet|Antiinfection Compound Library supplier|Antiinfection Compound Library in vitro|Antiinfection Compound Library cell line|Antiinfection Compound Library concentration|Antiinfection Compound Library clinical trial|Antiinfection Compound Library cell assay|Antiinfection Compound Library screening|Antiinfection Compound Library high throughput|Anti-infection Compound high throughput screening| in at least 50% of tumor cells (Fig. 2). The apoptotic index (AI) was expressed as the number of apoptotic tumor cells divided by the total number of tumor cells in the same field with evaluation of 1000 nuclei in randomly selected areas in each specimen (Fig. 3). Figure 1 Immunohistochemical staing for Cx26 in colorectal cancer. Cytoplasmic Cx26 expression was found (×200). Figure 2 Immunohistochemical staing for P53 in colorectal cancer. Nuclear
P53 expression was found in most tumor cells (×200). Figure 3 Apoptotic index (AI) as evaluated by TUNEL (×200). The slides were examined by two independent pathologists who were not aware of the corresponding clinicopathological data. Any cases with discordant scores were reevaluated JAK inhibitor a second time until a consensus was reached, no discrepancies between the evaluations were detected by the two
investigators. Statistical Analysis The data were compiled and analyzed using the SPSS software package for Windows (version 11.0; SPSS Inc., Chicago, Ill., USA). The relationship between Cx26 expression and the clinicopathological data, P53 and AI was evaluated by the chi-square test and Mann-Whitney U test. The disease specific survival was calculated by the Kaplan-Meier method and analyzed by the log-rank test. Prognostic factors were examined by univariate and multivariate analyses using a Cox proportional hazards model. P < 0.05 was considered to be significant. Results Cx26 expression was mainly localized in the cytoplasm of the cancer cells. In a few cases, we observed weak cytoplasmic staining in the normal mucosa. However
we did not consider this to be specific staining. Eighty-three of the 153 tumors (54.2%) showed Cx26 expression. TCL P53 expression was observed in 71 (46.4%). The correlation between Cx26 and the clinicopathological features is summarized in Table 1. Cx26 expression had a statistically significant relationship with disease recurrence and the histological type (P < 0.05). Moreover P53 expression had a statistically significant relationship with Cx26 expression (P < 0.05). The disease specific survival according to the status of Cx26 expression is shown in Fig.4. The patients with Cx26 negative tumors had significantly worse survival than those with positive tumors (P < 0.05). Cx26 expression was an independent prognostic factor, as well as lymph node metastasis, blood vessels invasion according to a multivariate analysis (Table 2). There was no significant correlation between Cx26 and AI (Fig. 5). Table 1 Correlation between the Cx26 expression and clinicopathological features Cx26 Negative Positive P-value Age (mean ± SD, years. 66.4 ± 8.1 66.4 ± 10.5 Gender Male 41 46 Female 29 37 0.