pallidum. The response of NK cells producing IFN-γ against microbial stimulation is highly significantly associated with KIR genotype [32]. Artavanis-Tsakonas et al. [23] reported that there was a significant association between KIR genotype and NK cell response to Plasmodium falciparum-infected erythrocytes and found that induction of IFN-γ synthesis https://www.selleckchem.com/products/bay-57-1293.html was dependent on the direct contact between NK cells and the infected erythrocytes. Therefore, we speculate that IFN-γ productions

in response to syphilis maybe have association with KIR genotype, but this needs to be confirmed further. Here, we put forward two hypotheses by which KIR genotype might affect NK cell producing IFN-γ in responses to syphilis. First, signals produced from the interaction of KIRs with their ligands during T. pallidum infection might modulate the degree of activation of NK cells. Alternatively, binding of KIR by relevant HLA ligands during NK cell ontogeny might lead to greater or lesser education of NK cells. Additional studies examining KIR–HLA class I interactions in patients and

controls may be fruitful. “
“Activation of NK cells is a hallmark of infections with intracellular pathogens. We previously showed that the BMS-777607 protozoan parasite Leishmania infantum triggered a rapid NK-cell response in mice that required TLR9-positive myeloid DC and IL-12, but no IFN-α/β. Here, we investigated whether IL-15 or IL-18 mediate the activity of IL-12 or function as independent activators of NK cells. In contrast to earlier studies that described IL-15 as crucial for NK-cell priming in response to TLR ligands, the expression of IFN-γ, FasL, perforin and granzyme B by NK cells in L. infantum-infected mice was completely preserved in the absence of IL-15, whereas the proliferative capacity of NK cells was lower than in WT mice. IFN-γ secretion, cytotoxicity ZD1839 research buy and FasL expression of NK cells from infected IL-18−/− mice were significantly reduced compared with controls, but, unlike IL-12, IL-18 was not essential for NK-cell effector functions.

Part of the NK-cell-stimulatory effect of IL-12 was dependent on IL-18. We conclude that IL-15 is not functioning as a universal NK-cell priming signal and that IL-18 contributes to the NK-cell response in visceral leishmaniasis. The cytokine requirements for NK-cell activation appear to differ contingent upon the infectious pathogen. “
“Tumour-loaded dendritic cells (DCs) from patients with chronic lymphocytic leukaemia (CLL) matured using an α-type 1-polarized DC cocktail (IL-1β/TNF-α/IFN-α/IFN-γ/poly-I:C;αDC1) were recently shown to induce more functional CD8+ T cells against autologous tumour cells in vitro than DCs matured with the ‘standard’ cocktail (IL-1β/TNF-α/IL-6/PGE2;PGE2DCs).