Joseph’s College (Autonomous), Tiruchirappalli, Tamil Nadu, India. DPPH was obtained from HiMedia laboratories Pvt. Ltd. Mumbai, India. All other chemicals used in this study were of analytical grade. About 5 g of fresh leaves were taken in a pre-weighed silica crucible. It was kept in air oven for an hour at 110 °C. Then the weight of the dry leaves was found out. From the difference in weight, the amount of water was determined. The ash content was determined by incineration of the dry plant sample in muffle furnace at 400 °C. About Selleck Rapamycin 0.5 g of ash was digested with con. HCl and the whole was dissolved in water and filtered. The filtrate

was made up to 100 mL in a standard flask. This made up solution was used for further analysis. The standard sodium ion solution was prepared (0.586 g NaCl in 100 mL). From the above solution, nine different concentration (1.0, 1.5, 2.0… 5.0 mL) were prepared. These solutions were taken for flame photometric studies (Systronics mediflame 127). A standard graph was plotted by taking concentration of sodium on the X-axis

and emission intensity shown by the flame photometric study on the Y-axis. Reading for the sample solution was fitted with the standard selleck chemicals graph. The percentage of sodium in plant sample was determined. The concentration of potassium and calcium were also calculated by the same procedure. The standard potassium (0.750 g KCl in 100 mL) and calcium solutions (0.55 g CaCl2 in 100 mL) were prepared. The determination of iron and phosphorous was done spectrocolorimetrically by standard graph method. The standard solutions of iron of different concentrations were prepared from the bulk solution (2.44 g of FAS in 250 mL). Each of the iron solution was treated with 4N HNO3 and NH4CNS. The percentage transmittance was measured at 470 nm. The nine different standard solutions of phosphorous were prepared from the bulk solution (0.1 g of KH2PO4 in 250 mL). Each of the phosphorus solution

Adenylyl cyclase was treated with ammonium molybdate and ammonium vanadate. The percentage transmittance was measured. Sulfur was also determined by spectrocolorimeter method. Unlike other method, the sulfur in plant sample was converted into sulfate using BaCl2. The concentration of copper, manganese and zinc in plants sample was determined by AAS (Atomic Absorption Spectrometer). A standard solution of Copper was prepared by dissolving 3.929 g of CuSO4.5H2O in 1000 mL of water and 10 mL of the solution was diluted to 100 mL with water. Standard solutions of Mn (3.076 g of Manganese sulfate in 1000 mL, treated with Nitric acid:perchloric acid (9:1) and Zn (4.398 g of Zinc sulfate in 1000 mL) were prepared. The determination of Cu, Mn and Zn was done by using AAS with the specifications for mono element hollow cathode lamp. The exact specifications should be as per the particular instrument used. The standard solution of magnesium was prepared by dissolving 3.076 g of MgSO4 in 1000 mL of deionized water.