g., injera). Furthermore, although fluid consumption in the present study was less than recommended [7], the daily total ad libitum water intake (0.23 ± 0.04 L/MJ) was consistent with guidelines from the
US National Research Council [33]. These guidelines suggest 1 mL of water per kcal (0.24 L/MJ) of EE for adults under average conditions of EE and environmental exposure with the rare exception of instructing the consumption of 1.5 mL/kcal (0.36 L/MJ) in cases of higher levels of physical activity, sweating and solute load. Additionally, the total water intake in the current study (3.2 L) is in accordance with optimal kidney function and urine output maintenance at high altitude (i.e., 3-4 L/day) [2]. This is also in agreement with the existing literature [8, 9, 18] where elite Kenyan distance runners maintained their hydration status due to the consumption of foods with a high quantity of water (e.g., ugali) [9]. On the other hand, fluid intake recommendations FHPI datasheet as set by the ACSM guidelines indicate that athletes should consume 5-7 mL/kg of BM of fluids at least 4 hours
prior to the exercise session aiming to start the physical activity euhydrated with normal plasma electrolyte levels [7]. Nevertheless, evidence to AZD1152 nmr support this recommendation is equivocal at this point. It is important to note that mild dehydration may actually be an advantage as, theoretically, it will lower the energy cost of running at the same relative intensity [34, 35]. Conclusions As previously found in elite Kenyan endurance runners, elite Ethiopian runners met dietary recommendations click here for endurance athletes for macronutrient intake but not for fluid intake. Nevertheless, it remains unclear how these differences in dietary patterns with regard to fluid consumption,
before major competitions, impact on their performance. Acknowledgements The PD-1 antibody cooperation of the subjects is greatly appreciated. We also thank Global Sports Communication http://www.globalsportscommunication.nl/ for their support and for allowing us to stay so close to these great athletes. Finally, we thank Thelma Polyviou for her help. References 1. IAAF.org Home of World Athletics [http://www.iaaf.org/mm/document/imported/38451.pdf] 2. Rodriguez NR, Di Marco NM, Langley S: American College of Sports Medicine position stand. Nutrition and athletic performance. Med Sci Sports Exerc 2009, 41:709–731.PubMedCrossRef 3. Friedman JE, Lemon PW: Effect of chronic endurance exercise on retention of dietary protein. Int J Sports Med 1989, 10:118–123.PubMedCrossRef 4. Gaine PC, Pikosky MA, Martin WF, Bolster DR, Maresh CM, Rodriguez NR: Level of dietary protein impacts whole body protein turnover in trained males at rest. Metabolism 2006, 55:501–507.PubMedCrossRef 5. Meredith CN, Zackin MJ, Frontera WR, Evans WJ: Dietary protein requirements and body protein metabolism in endurance-trained men. J Appl Physiol 1989, 66:2850–2856.PubMed 6.