Furthermore, STAT3 directly regulates fascin expression, and NF-κB binds to the fascin promoter in a STAT3-dependent and Notch-independent manner. Conclusion: Both STAT3
and NF-κB are required for fascin up-regulation, which is involved in cell migration and invasion in GC cells, and STAT3-NF-κB-fascin signaling axis is identified as a therapeutic target for blocking GC cell invasion and migration. Key Word(s): 1. gastric cancer; 2. fascin; 3. STAT3; 4. Notch; Presenting Author: CHUNXIAO CAI Additional Authors: YUANMING http://www.selleckchem.com/products/ink128.html ZHU, YUJUN ZHANG, YULAN LIU Corresponding Author: YULAN LIU Affiliations: Department of Gastroenterology, Peking University People’s Hospital Objective: Adiponectin receptor 2 (AdipoR2) plays important roles
during various http://www.selleckchem.com/products/epacadostat-incb024360.html tumorigenesis, through bound by its ligand. Previous work revealed that AdipoR2 was dysregulated in colorectal cancer. However, what the expression level AdipoR2 like in colorectal adenoma, the predisposing state of colorectal cancer, and whether AdipoR2 exert it effect on tumorigenesis via microRNA (miRNA) regulation are largely unknown. The aim of this study was to investigate the expression status of AdipoR2 in colorectal adenoma; and to explore whether some miRNAs target AdipoR2 and affect the biological behaviors of colorectal cancer. Methods: The expression status of AdipoR2 in different stages of colorectal adenomas were detected
by immunohistochemical staining, and the expression levels of the predictive miRNAs that target it in these adenomas were investigated by quantitative RT-PCR. AdipoR2 highly expressed colorectal cancer cell HCT-116 was used to establish these miRNAs stable transfected cell lines as test models. MTT, colony assay for cell viability, scratch assay for migration and Western blot analysis for AdipoR2 expression and apoptosis pathway related proteins were performed. Results: We found AdipoR2 expression level decreased in adenomas, especially in advanced adenomas. Meanwhile miR-200c and miR-200b, which selected by bioinformatic 上海皓元 analysis as the candidate miRNAs that target AdipoR2, demonstrated the opposite expression tendencies in different stages of adenoma towards AdipoR2. Enhanced miR-200c, miR-200b promoted cell proliferation, accelerated cell transit from G0/G1 phase to S phase, reduced apoptosis, and increased cell migratory ability by down-regulating AdipoR2 in vitro. Conclusion: AdipoR2 and miR-200c, miR-200b may be intimately related to the progression colorectal cancer, especially in early adenoma stage. Whether miR-200c, miR-200b could be use as novel targets for early detection and treatment still need further studies. Key Word(s): 1. AdipoR2; 2. miR-200c; 3. miR-200b; 4.