Disclosures: Stephen A. Harrison – Advisory Committees or Review Panels: Merck, Nimbus Discovery; Grant/Research Support: Merck, Genentech; Speaking and Teaching: Merck, Vertex Sheldon http://www.selleckchem.com/products/KU-60019.html Y. Okada – Employment: Gilead Sciences; Stock Shareholder: Gilead Sciences Cathy A. Su – Employment: Gilead Sciences, Inc.; Stock Shareholder: Gilead Sciences, Inc. Matthew Paulson – Employment: Gilead Sciences Jeffrey D. Bornstein – Employment: Gilead Sciences Arun J. Sanyal – Advisory Committees

or Review Panels: Bristol Myers, Gilead, Abbott, Ikaria; Consulting: Salix, Immuron, Exhalenz, Nimbus, Genentech, Echo-sens, Takeda; Grant/Research Support: Salix, Genentech, Genfit, Intercept, Ikaria, Takeda, GalMed, Novartis, Gilead; Independent Contractor: UpToDate, Elsevier [Objective] We reported the usefulness of cytokeratin18 (CK18) as an index of progress of the hepatic tissue in the example of a repetition liver biopsy of NASH.(Hepatology 474A,2013) .Recently, the novel sugar chain marker WFA+M2BP has attracted attention as a potentially useful non-invasive liver fibrosis marker, and reported high levels of WFA+M2BP is high risk of hepatocellur carcinoma(HCC) with hepatitis C, but its utility in NASH/NAFLD is unknown. Therefore, we have assessed WFA-M2BP in

NAFLD cases and examied its utility as a marker of fibrotic change in the diagnosis of progression of fibrosis and repeat liver biopsies and whether it could useful as prediction of HCC.[Subjects PI3K inhibitor and Methods] We assessed WFA-M2BP in 294 NAFLD patients at who had undergone liver biopsies (mean age: 54.2±14; M/F: 149/145; F0/1/2/3/4: 24/87/65/87/31). In addition to its utility in diagnosing fibrosis in NASH, we examined the associations

of WFA-M2BP to fibrosis stage. Moreover, M2BP of a HCC cases and non HCC cases was measured. We also assessed WFA-M2BP in 96 NAFLD patients (5.3±2.9y) who underwent repeat liver biopsies We examined the associations of WFA-M2BP to liver fibrosis changes. This kit measures gly-cosylation isomer of Mac-2 binding protein based on the che-miluminescence enzyme immunoassay method with CDP-StarR 上海皓元 chemiluminescent substrate. This kit is exclusively designed for Automated Immunoassay System HISCL- series. [Results] WFA-M2BP increased as fibrosis progressed (P<0.0001). Specifically, WFA-M2BP was significantly higher in F3 and F4 than in F0-2 (0.8 vs 1.23; P<0.0001), the cutoff value was 1.01, the AUC was 0.70, sensitivity was 68.4%, specificity was 70%, PPV was 60%, and NPV was 77.5%; thus, WFA-M2BP was useful in diagnosing NASH with progression of fibrosis. Among cirrhotic liver, WFA-M2BP was higher with Burn out NASH in which advanced fibrosis than non-burn out NASH(6.5 vs 1.8,p=o.0031). Furthermore the HCC cases(n=13)had significantly high levels of M2BP compared with the non HCC cas-es(n=281)(6.5 vs 1.8,p<0.0001).