By dividing the reaction into two stages, both the standard and the modified assays can be automated for high-throughput processing. Fig. 1 Reaction schemes for measuring the activities of RCA and Rubisco in continuous assays. The two diagrams show alternative pathways buy CX-6258 for coupling 3-PGA formation to NADH oxidation. a Pathway for measuring RCA activity. The coupling of 3-PGA formation to NADH oxidation is independent of adenine nucleotides, allowing measurement of RCA activity at variable ratios of ADP:ATP. b Pathway for measuring
Rubisco and Rubisco activation. The coupling of 3-PGA formation to NADH oxidation requires ADP Materials and methods Materials Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the United States Department
of Agriculture and does not imply its approval selleck products to the exclusion of other products or vendors that may also be suitable Biochemical reagents of the highest purity available were purchased from Sigma–Aldrich (St. Louis, MO, USA). Ribulose 1,5-bisphosphate was synthesized by isomerization and phosphorylation of ribose 5-phosphate (Jordan and Ogren 1984). Rubisco was purified from tobacco or Arabidopsis leaves as described previously and converted to the ER form (Carmo-Silva et al. 2011). Recombinant tobacco and Arabidopsis RCA was expressed in Escherichia coli and purified as described previously (van de Loo and Salvucci 1996; Barta et al. 2011). Plant material and conditions used for growth The conditions used for growth of Arabidopsis thaliana (L.) Heynh. wild type, cv. Columbia, and the transgenic
line rwt43 (Zhang et al. 2002) were described previously (Carmo-Silva and Salvucci 2013). Camelina (Camelina sativa (L.) Crantz cv. Robinson) and tobacco (Nicotiana tabacum L. cv. Petit Havana) plants, including transgenic tobacco plants that express a His-tagged Rubisco (Rumeau et al. 2004), were grown under the conditions described in Carmo-Silva and Salvucci (2012). Measurements were conducted on fully expanded leaves of 4–5 week old plants of Arabidopsis oxyclozanide and camelina, and 5–6 week old plants of tobacco. Isolation and expression of cDNAs and protein for dPGM and PEP carboxylase A cDNA clone for dPGM was isolated from E. coli (Fraser et al. 1999) and cloned into pET23a (Novagen, Madison, WI, USA). Nucleotides that encode for a C-terminal Strep-tactin (S-Tag) were added to the cDNA clone by PCR using a modified reverse primer. The modified primer encoded for the eight amino acid S-Tag (W-S-H-P-Q-F-E-K) that was linked to the authentic C-terminus by two amino acids; Ser-Ala. Recombinant dPGM protein containing the S-Tag (dPGM-ST) was expressed in E coli BL21Star™(DE3)pLysS as described by van de Loo and Salvucci (1996). Frozen cell pellets containing dPGM-ST were thawed in 0.