6–28.3 pg/mL) at 2 or 6 h and maintained a value lower than the 0 h level at 24 h (Fig. 2c). During the dosage period of 24 weeks, the intact PTH level decreased significantly at 12 and 24 weeks (Fig. 2d). Fig. 2 Mean changes in serum calcium and intact PTH after injection of 56.5 μg. teriparatide Time courses of corrected serum calcium (a) and intact PTH (c) over 24 h at 0 weeks (black circle), 4 weeks (white circle), 12 weeks (black triangle), and 24 weeks (white triangle),

and the changes in the baseline levels of corrected serum calcium (b) and intact PTH (d) over 24 weeks. Data are plotted as means (±SE) *p < 0.05 **p < 0.01 versus 0 h or 0 weeks with paired t test Twenty-four hour changes in bone turnover markers after each injection The 24 h percent changes in bone turnover markers after each teriparatide injection at #Selonsertib cell line randurls[1|1|,|CHEM1|]# each

data collection week are shown in Fig. 3. The serum osteocalcin level decreased to its minimum value (−9.8 to −17.5 %) at 6, 8, or 24 h (Fig. 3a). The levels at 24 h were mostly significantly lower than at 0 h. The serum P1NP decreased to its minimum value (−15.1 to −22.3 %) at 6 h and then increased significantly to about 5 % (4.9 to 8.6 %) at 24 h after the teriparatide injection (Fig. 3b). The urinary NTX increased to its maximum value (41.2 to 67.4 %) at 4 www.selleckchem.com/products/ew-7197.html or 6 h and then decreased (Fig. 3c). The DPD increased to its maximum value (29.5 to 31.6 %) at 2 or 4 h and then decreased significantly (Fig. 3d). The profiles of the 24 h changes in each bone turnover marker were almost the same in each collection week. Fig. 3 Mean percent changes from 0 to 24 h for serum osteocalcin (a), serum P1NP (b), urinary NTX (c), and urinary DPD (d) at 0 weeks (black circle), 4 weeks (white circle), 12 weeks (black triangle), and 24 weeks (white triangle). Data are plotted as means (±SE) *p < 0.05 **p < 0.01 versus 0 h with paired t test Changes in bone turnover marker levels over 24 weeks Percent changes from baseline for 24 weeks were calculated for serum osteocalcin and P1NP and urinary NTX and DPD. The serum osteocalcin levels before each teriparatide injection were significantly

increased by 26.8 % HAS1 at 4 weeks, and the levels were maintained for 24 weeks (Fig. 4a). The serum P1NP level increased significantly by 19.9 % at 4 weeks and then decreased to the baseline level at 12 weeks (Fig. 4b). The urinary NTX decreased significantly by 14.8 % at 4 weeks and subsequently returned to the baseline level (Fig. 4c). The urinary DPD decreased by 17.8 % at 4 weeks and then maintained this lower level (Fig. 4d). Fig. 4 Mean percent changes in 0 h values from 0 to 24 weeks for serum osteocalcin (a), serum P1NP (b), urinary NTX (c), and urinary DPD (d). Data are plotted as means (±SE) *p < 0.05 **p < 0.01 versus 0 week with paired t test Lumbar bone mineral density The percent change in lumbar BMD increased 2.6 % from baseline at 24 weeks. Safety No serious AEs were observed in this study. AEs occurred in 21 (75 %) subjects.