2 Adequacy of the genetic risk perception Overestimation 77 66.9 Adequate Estimation 30 26.1 Underestimation 8 6.9 *14 subjects were unable to report their risk levels for cancer of the breast and/or ovaries **15 subjects were unable to report their level of risk of being a carrier of the genetic mutation of the BRCA1 and BRCA2 genes Subjective and objective risk The mean percentage regarding the subjective risk of developing a tumour and of being a carrier of the genetic mutation were 39% and

40%, respectively. The mean percentage regarding the objective risk, calculated using the BRCAPRO model, of developing a tumour and of being a carrier of the genetic mutation were 11% and 19%, respectively. Anxiety and Depression The total mean score was 13, with 24% of the EPZ5676 concentration subjects suffering one episode of

major depression and 19% experiencing the presence of some disturbance in adaptation. A mean score of 8 was found for the single scales (borderline anxiety) and of 5 (normal depression). A total of 25% had borderline anxiety levels and the same value was found in subjects suffering from anxiety. Depression was found in 9% of the subjects, while 15% were borderline. Association between medico-demographic variables and Alpelisib risk perception (table 4 and 5) Table 4 Associations between the perception of risk (CRP-GRP) and Medical-Demographic variables   N Mean Std. Deviation P (2-tailed) YM155 ic50 ELIGIBILITY Cancer Risk Perception         Non-Eligible 44 32.82 21.87   Eligible 72 43.04 24.13 .024* Genetic Risk Perception         Non-Eligible 43 29.11 21.92   Eligible 72 46.45 21.96 .000* PATHOLOGY Janus kinase (JAK) Cancer Risk Perception         Non-Affected 84 38.63 21.14   Affected 32 40.89 30.35 .712 Genetic Risk Perception         Non-Affected 83 37.90 22.99   Affected 32 45.23 23.74 .108 Table 5 Associations

between the perception of risk (CRP-GRP) and Medical-Demographic and Psychological variables   Cancer risk perception Genetic risk perception Anxiety        Pearson coefficient 0.050 0.087    P (2-tailed) 0.596 0.355 Depression        Pearson coefficient -.031 .072    P (2-tailed) .742 .537 Age        Pearson coefficient -.068 -.030    P (2-tailed) .468 .747 Number of relatives affected by breast and/or ovarian cancer        Pearson coefficient .053 -.082    P (2-tailed) .569 .386 Number of relatives affected by other types of tumour        Pearson coefficient -.149 -.139    P (2-tailed) .111 .140 BRCA pro Cancer Risk        Pearson coefficient .254      P (2-tailed) .006 — BRCA pro Genetic Risk        Pearson coefficient   .322    P (2-tailed) — .000 Of all the medical-demographical variables, only the condition of eligibility was found to be statistically associated to the perception of risk (Table 4). The subjects who were eligible for genetic testing had a significantly higher perception of risk compared to the non-eligible people (CRP = 43%vs33%, p = 0.024; GRP = 46%vs29%, p < 0.000).