paratuberculosis K10 (AE016958.1), M. smegmatis MC2 155 (CP000480.1), M. abscessus ATCC 19977 (CU458896.1), M. gilvum PYG-GCK (CP000656.1), M. vanbaalenii PYR-1 (PLX3397 CP000511.1), Mycobacterium sp. JLS (CP000580.1), Mycobacterium sp. KMS (CP000518.1), Mycobacterium sp. MCS (CP000384.1), and DNA sequences of non-targeted genomes include Corynebacterium aurimucosum ATCC 700975 (CP001601.1), C. diphteriae NCTC 13129 (BX248353.1), C. efficiens YS-314 (BA000035.2), C. glutamicum ATCC 13032 (BX927147.1), C. jeikeium K411 (NC_007164), C. kroppenstedtii DSM 44385 (CP001620.1), C. urealyticum DSM 7109 (AM942444.1), Nocardia farcinica buy OICR-9429 IFM 10152 (AP006618.1),

Nocardioides sp. JS614 (CP000509.1), Rhodococcus erythropolis PR4 (AP008957.1), R. jostii RHA1 (CP000431.1) and R. opacus B4 (AP011115.1). Selection of exclusively conserved proteins in Mycobacterium spp. genomes Among the 3989 predicted proteins of M. tuberculosis H37Rv genome (Figure 2A and Additional file 1), about 54.6% (i.e. 2177 proteins) presented protein similarities above 50% with the other studied mycobacterial genomes (n = 15), and only 6.8% of these

hypothetical conserved mycobacterial proteins (150 proteins: 150 number in the top of a bar in Figure 2B) displayed similarities less than 50% with the studied non-mycobacterial genomes (n = 12). Consequently, almost half Target Selective Inhibitor Library supplier of the M. tuberculosis H37Rv predicted proteins are potentially present in the 12 studied genomes of CNM group members. We chose to decrease the number of candidate proteins by restricting the panel of studied proteins to those exclusively conserved

in the mycobacterial genomes, focusing on M. tuberculosis H37Rv proteins with similarity levels between 80% and 100% in comparison with other mycobacterial genomes (n = 15), and less than 50% similarity levels in comparison with genomes Fossariinae (n = 12) of the other CNM group genera. As a result, among the 3989 predicted proteins of M. tuberculosis H37Rv genome (Figure 2A), we selected 11 proteins (11 number in the top of a bar in Figure 2B). Among the 3989 predicted proteins of M. tuberculosis H37Rv proteins (Additional file 1), the selected candidate proteins (Table 1), were the subunits C (locus Rv1305) and A (locus Rv1304) of the ATP synthase, the cyclopropane mycolic acid synthase (CMAS) coded by the cmaA1 gene in M. tuberculosis H37Rv (locus Rv3392c), hypothetical PE or PPE family proteins (loci Rv0285 and Rv3022c), proteins coded by esxG, esxH and esxR genes in M. tuberculosis H37Rv (loci Rv0287, Rv0288, Rv3019c, respectively), and proteins such as a lipoprotein coding by lppM gene (locus Rv2172c), an oxidoreductase (locus Rv0197), and a small secreted protein (locus Rv0236A). Figure 2 Total (A) and partial representation (B) of the protein number (vertical axe, number in the top of the bars) of Mycobacterium tuberculosis H37Rv genome, according to their similarities with proteins of targeted mycobacterial genomes and proteins of non-targeted genomes (horizontal axes).